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以欧洲甜樱桃(Prunus aviumL.)幼胚子叶为试材,研究不同品种、不同子叶发育时期、6-BA和NAA配比和培养条件等对其离体再生不定芽的影响。结果表明,以MS为基本培养基,附加6-BA2.0mg.L-1+NAA0.2mg.L-1,用PF(子叶的长度/胚的长度×100)=50~80发育阶段的子叶,’那翁’品种幼胚子叶再生不定芽效果最好,再生率最高可达79.2%。暗培养2周后再转入光照下培养,对子叶再生不定芽具有一定的促进作用。’雷尼尔’品种子叶再生率为72.2%,’先锋’为61.1%,’拉宾斯’为33.3%。子叶再生不定芽全部发生于子叶正面近胚芽端切口处,表现出明显的极性效应。
The effects of 6-BA, NAA ratio and culture conditions on the adventitious buds regenerated from Prunus avium L. were studied in different varieties and different cotyledon developmental stages. The results showed that with MS as the basic medium, 6-BA 2.0 mg.L-1 + NAA 0.2 mg.L-1 was added, and the cotyledons with PF (cotyledon length / embryo length × 100) = 50-80 development stage , ’Weng’ varieties of young embryo leaves best adventitious bud regeneration, regeneration rate up to 79.2%. Dark culture for 2 weeks and then transferred to light culture, adventitious bud regeneration of cotyledon has a certain role in promoting. ’Rainier’ Cotyledon regeneration rate was 72.2%, ’pioneer’ was 61.1%, ’Labins’ was 33.3%. Cotyledon regeneration of adventitious buds all occurred in the cotyledon front near the embryo end incision, showing a significant polar effect.