Two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-

• 作 者: Qingming Shu Zhiqiang Li Shuwa
• 机 构: The Chinese PLA Postgraduate Medical School,Department of Pathology,Second Department of Internal Me
• 出 处: 中国神经再生研究（英文版）
• 发表日期: 2004年期
• 关键词: surface-enhanced laser desorption ionization-time of flight-mass spectrometry tw

Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou's weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.