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目的:研究促卵泡激素(FSH)结合片段对卵巢上皮性细胞癌细胞株hey增殖活性的影响。方法:应用卵巢上皮性细胞癌细胞株hey作为实验对象,分别加入FSH、FSH结合片段、FSH+FSH结合片段。用四甲基偶氮唑蓝(MTT)法检测卵巢癌hey细胞的生长状况,Western blot检测cyclinD1、Akt、pAkt分子的表达。结果:FSH对卵巢癌hey细胞的生长有明显的促进作用,细胞生长活性提高21%。FSH结合片段对卵巢癌细胞的生长有明显抑制作用,对细胞平均抑制率为22%,且能下调cyclinDI的表达,在2.5×10~(-9)Mol/L达70%。FSH结合片段对FSH有竞争抑制作用,细胞抑制率为18%,能抑制FSH上调cyclinD1的作用,抑制率为61%。FSH能上调pAkt的表达,对Akt的表达没有明显影响,在40mIU/ml的浓度时pAkt/Akt上调率达224%。FSH结合片段对Akt的表达没有明显影响,但能明显下调pAkt的表达,且呈时间依赖性(在1 5分钟时达66%)和剂量依赖性(在2.5×10~(-9)Mol/L达31%)。FSH结合片段能抑制FSH上调pAkt的作用,抑制率为80%。结论:FSH结合片段可通过抑制FSH诱导的PI3K/Akt-cycl-inD1信号通路进而抑制上皮性卵巢癌hey细胞的增殖活性。
Objective: To study the effect of follicle stimulating hormone (FSH) binding fragment on hey proliferative activity of epithelial ovarian cancer cell line. Methods: Epithelial ovarian cancer cell line hey was used as experimental subjects, and FSH, FSH-binding fragment and FSH + FSH-binding fragment were respectively added. The growth of ovarian cancer hey cells was detected by MTT assay. The expressions of cyclinD1, Akt and pAkt were detected by Western blot. Results: FSH significantly promoted the growth of ovarian cancer hey cells and the cell growth activity increased by 21%. The FSH binding fragment could significantly inhibit the growth of ovarian cancer cells, with an average inhibition rate of 22% and a decrease of cyclinDI expression of 70% at 2.5 × 10 -9 mol / L. FSH binding fragment competitive inhibition of FSH, the cell inhibition rate was 18%, can inhibit FSH upregulation of cyclinD1, the inhibition rate was 61%. FSH up-regulated the expression of pAkt, but had no obvious effect on the expression of Akt. The up-regulation rate of pAkt / Akt reached 224% at the concentration of 40mIU / ml. FSH binding fragment had no significant effect on the expression of Akt, but significantly down-regulated the expression of pAkt in a time-dependent manner (66% at 15 min) and in a dose-dependent manner (2.5 × 10 -9 mol / L up to 31%). FSH binding fragment can inhibit FSH upregulation of pAkt role, the inhibition rate was 80%. Conclusion: The FSH-binding fragment can inhibit the proliferation of epithelial ovarian cancer hey cells by inhibiting the PI3K / Akt-cycl-inD1 signal pathway induced by FSH.