论文部分内容阅读
目的观察严重烫伤小鼠小肠集合淋巴结(Peyer patches,PP结)细胞凋亡的变化规律,探讨其在肠黏膜免疫屏障功能障碍中的作用。方法将40只BALB／c小鼠随机分为正常对照组及烫伤后12、24、72 h组,每组10只。各致伤组小鼠背部造成20％TBSAⅢ度烫伤后,按时相点处死并留取全部PP结。对照组小鼠处死后亦留取相同标本。分别行HE染色、DNA琼脂糖凝胶电泳,标记PP结细胞作流式细胞仪(FCM)检测。结果HE染色见烫伤小鼠PP结生发中心有大量淋巴细胞凋亡。DNA琼脂糖凝胶电泳显示烫伤组各时相点有“梯形”条带。FCM结果显示对照组和烫伤各组PP结细胞凋亡率分别为(4．9±2．1)％、(26．7±3．1)％、(21．6±4．0)％、(12．8±2．0)％,伤后12 h为峰值。结论严重烫伤小鼠PP结淋巴细胞早期凋亡为细胞的主要死亡模式,在严重烫伤后早期肠黏膜免疫屏障功能损伤中起重要作用。
Objective To observe the changes of apoptosis in the small intestine-collecting lymph nodes (Peyer patches) of severely scalded mice and to explore its role in the immunologic barrier dysfunction of the intestinal mucosa. Methods Forty BALB / c mice were randomly divided into normal control group and 12, 24, 72 hours after scald. Each injury group mice back caused 20% TBSA Ⅲ degree scald, according to the time point of death and take all PP knot. Control mice were sacrificed after taking the same specimen. HE staining, DNA agarose gel electrophoresis, labeling of PP-labeled cells for flow cytometry (FCM) detection. Results Hematoxylin and eosin staining showed that there was a large number of lymphocyte apoptosis in the center of PP-induced gerbils in scalded mice. DNA agarose gel electrophoresis showed a “trapezoidal” band at each time point of the scald group. The results of FCM showed that the percentage of apoptotic cells in control group and scalded group were (4.9 ± 2.1)%, (26.7 ± 3.1)% and (21.6 ± 4.0)% respectively, (12.8 ± 2.0)%, peaked 12 h after injury. Conclusion The early apoptosis of PP-knot lymphocytes in severe scalded mice is the major cell death pattern, which plays an important role in the early damage of intestinal mucosal immune barrier after severe scald.