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目的建立适于MR I研究的鼠脑胶质瘤模型。方法采用脑立体定向术,在F ischer344大鼠脑内接种F98胶质瘤细胞。接种后观察大鼠的生存期并进行生存分析。接种后第6、8、10、12、14天采用MR I及病理学方法动态观察肿瘤生长情况。结果接种成功率为100%,生存分析表明接种后老鼠多数于14 d左右死亡。接种后6 d,MR I即可检测出肿瘤生长。随着接种时间延长,肿瘤体积增大,接种后第6、8、10、12、14天组织学测量分别为(3.6±0.2)mm3、(8.1±0.5)mm3、(63.2±0.6)mm3、(127.3±0.8)mm3,(312.6±0.7)mm3。MR I测量的肿瘤体积为(4.1±0.3)mm3、(8.5±0.4)mm3、(65.4±0.7)mm3、(132.5±0.2)mm3、(317.8±1.4)mm3。组织学测量与MR I体积测量差异无统计学意义(t=0.943~2.287,P值均>0.05),但两者明显相关(r=0.91,P<0.01)。第10、12、14天组肿瘤体积较第6、8天组明显增大(F=3.52,P<0.05)。病理学显示该肿瘤模型具有胶质瘤的病理特点。结论该胶质瘤模型成瘤率高,成瘤快,有良好的可预测性和可重复性,是适合MR I研究的理想模型。细胞接种后10~14 d是最佳的研究时期。
Objective To establish a murine glioma model suitable for MR I studies. Methods F98 glioma cells were inoculated intracerebrally into Fischer344 rat brain by stereotactic technique. After inoculation, the survival of the rats was observed and the survival analysis was performed. On the 6th, 8th, 10th, 12th and 14th day after inoculation, the tumor growth was observed dynamically by MR I and pathology. Results The inoculation success rate was 100%. Survival analysis showed that most mice died after 14 days of inoculation. Six days after inoculation, tumor growth was detected by MRI. With the prolongation of inoculation time, the volume of tumor increased and the histological measurements were (3.6 ± 0.2) mm3, (8.1 ± 0.5) mm3, (63.2 ± 0.6) mm3, (127.3 ± 0.8) mm3, (312.6 ± 0.7) mm3. The tumor volumes measured by MR I were (4.1 ± 0.3) mm3, (8.5 ± 0.4) mm3, (65.4 ± 0.7) mm3, (132.5 ± 0.2) mm3, and (317.8 ± 1.4) mm3, respectively. There was no significant difference between histological measurement and MRI volume measurement (t = 0.943 ~ 2.287, P> 0.05), but both were significantly correlated (r = 0.91, P <0.01). The tumor volume on the 10th, 12th and 14th day was significantly higher than that on the 6th and 8th days (F = 3.52, P <0.05). Pathology shows that the tumor model has the pathological features of glioma. Conclusion The glioma model has high rate of tumorigenesis, rapid tumorigenesis, good predictability and repeatability, which is an ideal model for MRI research. 10-14 days after cell inoculation is the best study period.