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利用抗8氮鸟嘌呤的F9-1 aza突变株细胞与大鼠胸腺细胞融合,在HAT培养基中成功地筛选到FRT杂交细胞。该种细胞与亲本细胞F9-1 aza的细胞形态完全不同,呈成纤维样、上皮样、圆形或多突起样的分化细胞形态。在扩大繁殖后主要为成纤维样或上皮样细胞。对其中一个集落FRT-1核型检查表明杂种细胞染色体众数为80(范围62—88),C分带显示??包含F9和大鼠胸腺细胞二个亲本的染色体。FRT-1细胞的乳酸脱氢酶图谱中出现由大鼠LDH-A亚基与小鼠LDH-A亚基组成的杂种酶带。该细胞接种到经x光照射的129/Sv-ter小鼠皮下不能长瘤,亦不能在软琼脂上生长,表明F9-1 aza细胞的恶性表型已受到大鼠胸腺细胞基因组的抑制。
F9-1 aza mutant cells using anti-8-azaguanine were fused with rat thymocytes, and FRT hybrid cells were successfully screened in HAT medium. This kind of cells and the parental cells F9-1 aza completely different cell morphology was fibroblast-like, epithelial like, round or more protruding like cell morphology. Mainly fibroblast-like or epithelioid cells after enlarged reproduction. Karyotyping of FRT-1 in one of the colonies showed that the chromosomal size of the hybrid cells was 80 (range 62-88) and the C banding showed that the chromosomes of both parents of F9 and rat thymocytes were included. The lactate dehydrogenase pattern of FRT-1 cells shows a hybrid band consisting of the rat LDH-A subunit and the mouse LDH-A subunit. Inoculation of xenografted 129 / Sv-ter mice with xenografts failed to develop tumors and failed to grow on soft agar, indicating that the malignant phenotype of F9-1 aza cells has been inhibited by the rat thymocyte genome.