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作者用纯净的溶组织内阿米巴感染小鼠,首创性地建立了肠道阿米巴病的小鼠模型。在此基础上,作者用9个纯系和1个远交系的不同品系小鼠作材料,研究了遗传控制小鼠感染溶组织内阿米巴的易感性。建立动物模型所用的无菌溶组织内阿米巴为HM-1-L_3亚株,是将HM-1株阿米巴3次通过仓鼠肝脏而分离出来的一个毒性亚株。在盛有FYI-S-33培养液的250ml培养瓶中,于37℃培养3天后,取瓶中的阿米巴悬液,于400g离心10分钟,计数后用培养液稀释到所需浓度,即可用于接种。接种的具体步骤如下:先将不带鼠内阿米巴(E.
The authors infected mice with pure Entamoeba histolytica and pioneered the development of a murine model of intestinal amoebiasis. On this basis, the authors used nine different lines of purebred and one outbred strains of mice as materials to study the genetic control of mice infected with E. histolytica susceptibility. The aseptic tissue of Entamoeba histolytica used in animal model was HM-1-L_3 sub-strain, a toxic sub-strain isolated from the hamster liver by three times of HM-1 amoeba. After incubating at 37 ° C for 3 days in a 250 ml culture flask containing FYI-S-33 broth, the ampicillin suspension in the flask was centrifuged at 400 g for 10 minutes and then diluted to the desired concentration with the culture broth after counting. Can be used for inoculation. Vaccination specific steps are as follows: first without E. ameba (E.