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目的：观察ＭｇＳＯ４作为非竞争性ＮＭＤＡ－Ｒ拮抗剂对大鼠脑缺血－再灌注的保护作用。方法：用线栓法制作７２只Ｗｉｓｔａｒ大鼠的大脑中动脉栓塞模型，在术后２小时给予再灌注，分别在再灌注同时及其２小时后给予不同剂量ＭｇＳＯ４治疗。术后进行皮层脑电图描记，测定血Ｍｇ２＋浓度，进行运动功能评估，测定脑组织含水量，观察病理组织学变化，并计算凋亡细胞数。结果：ＭｇＳＯ４能减少扩展性抑制波产生救目（Ｐ＜０．０５）；１０％ＭｓＳＯ４组疗效优于５％ＭｇＳＯ４组，并明显改善大鼠运动功能，且早期给药效果更佳（Ｐ＜０．０５）；ＭｇＳＯ４能减轻脑组织含水量；但在短期内不能减少凋亡细胞数。结论：通过电生理、病理研究和临床评估证明ＭｇＳＯ４对大鼠脑缺血－再灌注损伤确有保护作用。
Objective: To observe the protective effect of MgSO4 as a non-competitive NMDA-R antagonist on cerebral ischemia-reperfusion in rats. Methods: The middle cerebral artery occlusion model of 72 Wistar rats was established by thread occlusion. The rats were reperfused 2 hours after the operation. Different doses of MgSO4 were given respectively at the same time and two hours after reperfusion. Cortical EEG was performed postoperatively to measure the serum Mg2 + concentration. The motor function was assessed. The water content of brain tissue was measured. The histopathological changes were observed and the number of apoptotic cells was calculated. Results: MgSO4 could reduce the rescuing wave (P <0.05). The effect of 10% MsSO4 group was better than that of 5% MgSO4 group, and significantly improve the motor function of rats, and the effect of early administration was better (P < 0.05); MgSO4 can reduce the water content of brain tissue; however, it can not reduce the number of apoptotic cells in a short time. Conclusion: MgSO4 has protective effect on cerebral ischemia-reperfusion injury in rats by electrophysiological, pathological and clinical evaluation.