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花药接种前麦穗置- 20 °C、10 m in 预处理有利于出愈和绿苗分化。花药接种后37 °C、8 h 培养使出愈率由8.2%(对照) 提高到29.0% 。在诱导培养基上附加2 m g/L的水杨酸 (SA) 对愈伤组织分化有促进作用, 而不影响出愈和绿苗分化。诱导培养基上附加0.02 m g/L表-油菜素内酯 (epi-BR) 能使绿苗分化率由25.7% (对照) 提高到47.2% , 白苗分化率由31.4% 降低到24.3% , 当epi-BR浓度提高到0.2 m g/L时, 却对绿苗和白苗分化均表现抑制作用。
Pre-anther inoculation - 20 ° C, 10 m in pre-treatment is conducive to the emergence and differentiation of green shoots. After anther inoculation 37 ° C, 8 h culture so that the outbreak rate increased from 8.2% (control) to 29.0%. The addition of 2 mg / L salicylic acid (SA) to the induction medium can promote the differentiation of callus without affecting the differentiation and differentiation of green shoots. Addition of 0.02 mg / L epi-BR to the induction medium increased the rate of green plantlet differentiation from 25.7% (control) to 47.2% and the rate of white plantlet differentiation from 31.4% to 24.3% When the concentration of epi-BR was increased to 0.2 mg / L, the differentiation of green and white seedlings was inhibited.