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配制普通小麦-大赖草Lr2、Lr7、Lr14 染色体二体异附加系(92G169、93G52-8、94G15) 与普通小麦-簇毛麦6VS/6AL易位系 (92R149) 3 个杂交组合, F1 分别与扬麦5 号正反杂交和自交。对正反杂交后代和F2 代个体进行有丝分裂中期染色体C-分带, 分析大赖草Lr2、Lr7和Lr14 染色体在F1 代的传递行为。结果为: Lr2随雄配子和雌配子的传递频率分别为27.3% 和17.9% , 在92G169×92R149F2 群体中,Lr2 染色体单体添加个体占29.9% ,二体添加个体占4.3% ; Lr7随雄配子和雌配子的传递频率分别为28.6% 和15.6% , 在 93G52-8×92R149F2 群体中, Lr7 染色体单体添加个体占30.1% , 二体添加个体占4.3% ; Lr14 随雄配子和雌配子的传递频率分别为71.4% 和29.5% , 在94G15×92R149F2 群体中, Lr14 染色体单体添加个体占59.8% , 二体添加个体占20.5% 。表明大赖草Lr2、Lr7、Lr14染色体在小麦背景中的传递行为存在较大差异, 三条染色体随雄配子的传递频率均高于随雌配子的传递频率, 其中Lr14 染色体随雄配子有优先传递的可能。
Three crosses were made between common wheat-Leymus racemosus Lr2, Lr7 and Lr14 chromosomal DNA (92G169,93G52-8,94G15) and common wheat-cluster wool 6VS / 6AL translocation line (92R149) Positive and negative hybridization with Yangmai 5 and selfing. The mitotic metachromosome C-banding was performed on the positive and negative hybrids and the F2 individuals. The transmission of Lr2, Lr7 and Lr14 chromosomes in F1 was analyzed. The results showed that the transmission frequency of Lr2 with male gametes and female gametes was 27.3% and 17.9%, respectively. Among the 92G169 × 92R149F2 populations, 29.9% of the individuals with Lr2 chromosomes and 4.3% And female gametes were 28.6% and 15.6%, respectively. In 93G52-8 × 92R149F2 population, individuals with Lr7 chromosome accounted for 30.1% and individuals with two bodies accounted for 4.3%. Lr14 passed with male gametes and female gametes The frequency was 71.4% and 29.5%, respectively. Among the 94G15 × 92R149F2 population, 59.8% of the individuals were individuals with Lr14 chromosomes and 20.5% of those with duodenums. The results showed that the transmission of Lr2, Lr7 and Lr14 chromosomes in the background of wheat was different. The transmission frequency of the three chromosomes with the male gametes was higher than that of the male gametes. The Lr14 chromosome was preferentially transferred with the male gametes may.