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本文构建了一种基于酶聚合体信号放大的多通道电化学免疫传感体系,并用于肝癌肿瘤标记物甲胎蛋白(AFP)的定量检测.该传感体系由固定了抗AFP鼠单克隆抗体的多通道丝网印刷电极组成,可捕获肿瘤标记物抗原AFP,进而与抗AFP兔多抗特异性结合形成夹心免疫复合物,然后利用辣根过氧化物酶聚合体偶联的羊抗兔二抗(Ig G-poly HRP)与三明治夹心免疫复合物结合,实现电流信号放大.该体系结合多通道丝网印刷电极及自主研发的多通道电化学检测仪,可同时满足多通道电流信号的检测.在最优化条件下,该传感体系检测AFP浓度的动态范围为64 pg·m L-1~250 ng·m L-1,最低检测下限为56 pg·m L-1,具有检测灵敏度高、特异性强、操作简便以及仪器便携等优点.
This article constructed a multi-channel electrochemical immunosensor system based on signal amplification of enzyme polymer and was used for the quantitative detection of liver cancer tumor marker AFP.The sensor system consisted of immobilized anti-AFP mouse monoclonal antibody Multi-channel screen printed electrodes, which capture the tumor marker antigen AFP, which in turn, specifically binds to anti-AFP rabbit polyclonal antibodies to form a sandwich immune complex and then uses horseradish peroxidase-conjugated goat anti-rabbit II Anti-Ig G-poly HRP combined with sandwiched sandwich immune complexes to amplify the current signal. This system combined with multi-channel screen printed electrodes and self-developed multi-channel electrochemical detector can simultaneously detect multi-channel current signals Under the optimized conditions, the dynamic range of AFP concentration was 64 pg · m L-1 ~ 250 ng · m L-1 and the lowest detection limit was 56 pg · m L-1, which had high detection sensitivity , Specificity, ease of operation and the advantages of portable equipment.