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在已育成的“异质同核系”,C103-cms-T,C103-cms-S,C103-cms-C,C103-cms-17A,C103-cms-19A,C103-cms-21A,C103-cms-22A和C103正常系的基础上作花药生化分析,发现各型不育系在生化成分上与正常系比较起来,含有较多的天门冬氨酸,较少的蛋氨酸、赖氨酸和脯氨酸;在α-淀粉酶、酯酶、过氧化物酶同工酶上也有明显的不同.恢复性遗传测验表明,以N6,74-20,525三个系依次测交,可区分T,S(M),C,21A型。此外,还观察到遗传测定与生化分析资料间的一些对应关系。生化分析方法可能作为区分不育类型的遗传测定法的补充手段。
C103-cms-T, C103-cms-S, C103-cms-C, C103- cms- 17A, C103- cms- 19A, C103- cms- 21A, C103- cms-22A and C103 on the basis of the normal line for anther biochemical analysis showed that each type of CMS biochemical composition compared with the normal line, containing more aspartic acid, less methionine, lysine and preserved There was also a significant difference in α-amylase, esterase and peroxidase isozymes.The recurrent genetic tests showed that the three lines N6,74-20,525 were sequenced to distinguish T, S ( M), C, 21A type. In addition, some correspondence between genetic assays and biochemical analysis data was also observed. Biochemical analysis may serve as a complementary measure of genetic testing to distinguish types of infertility.