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研究中国成都地区汉族成人迟发自身免疫性糖尿病(LADA)患者中人类白细胞抗原(HLA)A0205、A30基因频率分布,探讨它们与LADA的关系。选取成都地区符合纳入标准的LADA组患者41例(男25例,女16例,年龄52.17±11.56岁),2型糖尿病(T2DM)组患者40例(男26例,女14例,年龄60.69±12.20岁),正常对照(NC)组40例(男15例,女25例,年龄44.69±13.29岁)。应用巢式聚合酶链反应-序列特异引物(nested PCR-SSP)结合测序技术,采用2检验(双侧α=0.05),对三组研究对象分别比较了HLA-A0205、HLA-A30基因频率分布。121例DM及正常对照中,检出HLA-A0205 2例,LADA组及NC组各1例,等位基因频率分别占2.44%和2.5%;HLA-A30共5例,LADA组2例,T2DM组2例,NC组1例,等位基因频率分别占4.87%、5.0%和2.5%。均无统计学意义差异(P>0.05)。提示HLA-A0205、HLA-A30在中国成都地区汉族人中分布频率低,各组间分布差异无统计学意义。尚不能说明HLA-A0205、HLA-A30与中国成都地区汉族LADA有关。本研究样本较少,这一结论尚需进一步扩大样本,最好行家系连锁分析验证。
To investigate the frequency distribution of human leukocyte antigen (HLA) A0205 and A30 genes in adults with late-onset autoimmune diabetes (LADA) in Chengdu, China, and to explore their relationship with LADA. Forty-one patients (25 males and 16 females, aged 52.17 ± 11.56 years) with type 2 diabetes mellitus (T2DM) were enrolled in Chengdu LADA group, 40 males (26 males and 14 females, aged 60.69 ± 12.20 years old), 40 cases of normal control (NC) group (15 males and 25 females, age 44.69 ± 13.29 years). A nested PCR-SSP method was used to compare the frequency distribution of HLA-A0205 and HLA-A30 genes in three groups of subjects by 2 test (double α = 0.05) . In 121 cases of DM and normal controls, 2 cases of HLA-A0205, 1 case of LADA group and 1 case of NC group were detected, allele frequencies were 2.44% and 2.5%, 5 cases of HLA-A30, 2 cases of LADA group, 2 cases of T2DM Group 2 cases, NC group 1 case, the allele frequency accounted for 4.87%, 5.0% and 2.5%. There was no significant difference (P> 0.05). These results suggest that the frequency of HLA-A0205 and HLA-A30 is low in Han Chinese in Chengdu, China, with no significant difference among the groups. It can not explain HLA-A0205, HLA-A30 Han Chinese LADA in Chengdu, China. The sample of this study is less, this conclusion still needs to further expand the sample, the best expert system chain analysis and verification.