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OBJECTIVE To investigate whether nimesulide can suppress tumor growth and induce apoptosis in SGC-7901 gastric cancer cells and to explore the molecular mechanism involved. METHODS SGC-7901 cells were cultured in RPMI 1640 medium con- taining different concentrations of nimesulide (0,12.5, 50, 100, 200, 400 Mmol/L). The MTT assay, morphological observation, electron microscopy (EM), immunohistochemical analysis and Western blot analysis were em- ployed to investigate the effects of nimesulide on the SGC-7901 cells and to explore possible related molecular mechanisms. RESULTS Nimesulide inhibited the growth of SGC-7901 cells and elicit- ed typical apoptotic morphologic changes. Nimesulide also decreased NF-κB and Bcl-2 expression, but increased the level of the Bax protein. The positive rate of Bcl -2 protein expression at 0, 50, 100 and 200 μmol/L of nimesulide was 58.3±14.0%, 50.2±9.9%, 32.8±5.0% and 22.7± 5.5% respectively based on immunohistochemical staining. The positive rate of Bax protein expression was 22.0 ±5.7%, 29.2 ±6.5%, 42.7 ±5.9% and 74.5±9.1% and the NF-κB expression was 74.2+10.9%, 61.8±7.6%, 36.7 ±10.9% and 17.5 ±12.3%, Significant differences were found be- tween 0 μmol/L and 100 μmoI/L and 200 μmol/L. Western blot analysis al- so showed that the expression of NF-κB was decreased. CONCLUSION Nimesulide suppresses tumor growth and induces apop- tosis by inhibiting NF-κB expression, which may be related to the over- expression of Bax relative to Bcl-2 expression.
OBJECTIVE To investigate whether nimesulide can suppress tumor growth and induce apoptosis in SGC-7901 gastric cancer cells and to explore the molecular mechanism involved. METHODS SGC-7901 cells were cultured in RPMI 1640 medium con- taining different concentrations of nimesulide (0, 12.5, 50, 100, 200, 400 Mmol / L) The MTT assay, morphological observation, electron microscopy (EM), immunohistochemical analysis and Western blot analysis were em- ployed to investigate the effects of nimesulide on the SGC-7901 cells and to explore RESULTS Nimesulide inhibited the growth of SGC-7901 cells and elicit- ed typical apoptotic morphologic changes. Nimesulide also decreased NF-κB and Bcl-2 expression, but increased the level of the Bax protein. The positive rate of Bcl -2 protein expression at 0, 50, 100 and 200 μmol / L of nimesulide was 58.3 ± 14.0%, 50.2 ± 9.9%, 32.8 ± 5.0% and 22.7 ± 5.5% respectively based on immunohistochemical staining. The positive rat e of Bax protein expression was 22.0 ± 5.7%, 29.2 ± 6.5%, 42.7 ± 5.9% and 74.5 ± 9.1% and the NF-κB expression was 74.2 + 10.9%, 61.8 ± 7.6%, 36.7 ± 10.9% and 17.5 ± 12.3 Significant differences were found in be tween 0 μmol / L and 100 μmol / L and 200 μmol / L. Western blot analysis al-so showed that the expression of NF-κB was decreased. CONCLUSION Nimesulide suppresses tumor growth and induces apop- tosis by inhibiting NF-κB expression, which may be related to the over-expression of Bax relative to Bcl-2 expression.