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目的观察黄连提取物小檗碱(BBR)对溃疡性结肠炎(UC)模型小鼠结肠组织claudin-1蛋白及m RNA表达的影响。方法采用右旋葡聚糖硫酸钠(DSS)建立UC小鼠模型,将60只BALB/c小鼠随机分为空白对照组、模型对照组、BBR低剂量(50 mg/kg)组、BBR高剂量(100 mg/kg)组和柳氮磺吡啶(30 mg/kg SASP)阳性对照组。观察小鼠精神状况、体重等一般情况;评估疾病活动指数(DAI)、结肠大体形态损伤指数(CMDI)和结肠组织损伤指数(TDI);苏木精-伊红染色观察结肠组织的病理学变化;免疫组化SP法和RT-PCR法检测claudin-1蛋白及m RNA表达。结果与模型对照组相比,各治疗组小鼠结肠炎的临床表现和组织病理表现均有不同程度改善,其中BBR高剂量组和阳性对照组的DAI、CMDI和TDI评分均下降(P<0.01)。5组小鼠claudin-1蛋白m RNA相对表达量分别为1.00±0.03、0.12±0.02、0.21±0.02、0.63±0.03和0.81±0.03。模型对照组和各治疗组小鼠claudin-1蛋白和m RNA表达水平均较空白对照组小鼠降低(P<0.01),经BBR和SASP治疗后表达水平均较模型对照组升高(P<0.01),但BBR低、高剂量组claudin-1蛋白和m RNA的表达水平均低于阳性对照组(P<0.01)。结论 BBR能够通过上调紧密连接蛋白claudin-1的表达,有效缓解结肠炎症,但其治疗效果较SASP差。
Objective To observe the effect of berberine extract (BBR) on the expression of claudin-1 protein and m RNA in colonic mucosa of ulcerative colitis (UC) mice. Methods UC mouse models were established by dextran sodium sulfate (DSS). Sixty BALB / c mice were randomly divided into blank control group, model control group, BBR low dose (50 mg / kg) group, high BBR Dose (100 mg / kg) group and sulfasalazine (30 mg / kg SASP) positive control group. The mental status and body weight of the mice were observed. The disease activity index (DAI), gross morphological damage index (CMDI) and colon tissue injury index (TDI) were evaluated. The pathological changes of colon tissues were observed by hematoxylin-eosin staining Immunohistochemical SP method and RT-PCR method were used to detect the expression of claudin-1 protein and m RNA. Results Compared with the model control group, the clinical manifestations and histopathological changes of colitis in each treatment group improved to some extent. The scores of DAI, CMDI and TDI in BBR high dose group and positive control group decreased (P <0.01 ). The relative expression levels of claudin-1 protein m RNA in the 5 groups were 1.00 ± 0.03, 0.12 ± 0.02, 0.21 ± 0.02, 0.63 ± 0.03 and 0.81 ± 0.03, respectively. Compared with the blank control group, the expression of claudin-1 protein and m RNA in the model control group and each treatment group was lower than that in the blank control group (P <0.01), and the expression levels of claudin-1 and BBV were higher than those in the model control group (P < 0.01). However, the expression levels of claudin-1 protein and m RNA in low and high BBR groups were lower than those in positive control group (P <0.01). Conclusion BBR can effectively relieve colonic inflammation by up-regulating the expression of claudin-1, but its therapeutic effect is worse than that of SASP.