论文部分内容阅读
诊断真菌性角膜溃疡实验室通常采用Giemsa 染色法。但这种方法需要一定实践经验且据国外报导对真菌的检出率仅40%,Arffa 等人报告墨水——氢氧化钾湿片法(I-P 法)的检出率比Giemsa 法高的多,且方法简单,易于普及。但国内有作者报告不能重复出Arffa 等人的结果。为此我们做动物试验将I—P 法与Giemsa 法比较以检查I—P 法的应用价值。材料和方法一、试剂:①蓝黑墨水(北京牌)、②氢氧化钾2级(北京产),③Giemsa 粉(进口分装)、④醋酸氢化泼尼松注射液5ml/125mg。(浙江仙居制药厂)二、动物来源:新西兰白兔来自首都医学院动物实验中心,重量为1.5—2kg,共5只。三、真菌及菌液的制备:茄病镰刀菌是
Diagnostic fungal corneal ulcer laboratories usually use Giemsa staining. However, this method requires some practical experience and according to foreign reports, the detection rate of fungi is only 40%. Arffa et al. Report that the detection rate of ink-potassium hydroxide wet method (IP method) is much higher than Giemsa method, The method is simple and easy to popularize. However, some domestic authors report that the results of Arffa et al. Can not be repeated. For this purpose, we conducted animal experiments to compare the I-P method with the Giemsa method to examine the value of the I-P method. Materials and methods First, the reagents: ① blue black ink (Beijing card), ② potassium hydroxide 2 (Beijing production), ③ Giemsa powder (imported sub-packaging), ④ prednisone acetate injection 5ml / 125mg. (Zhejiang Xianju Pharmaceutical Factory) Second, animal origin: New Zealand white rabbits from the Capital Medical Animal Experimental Center, weighing 1.5-2kg, a total of five. Third, the preparation of fungi and bacteria: Fusarium solani is