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目的建立一种亚麻木酚素-开环异落叶松树脂酚二葡萄糖苷(SDG)的实验室提取和检测方法。方法在SDG的提取过程中对提取步骤的每一个环节进行单因素实验,观察不同提取方法、溶液及浓度、时间及碱解方法对亚麻籽SDG提取率的影响,用高效液相色谱测定SDG的含量。结果 3种不同提取方法的SDG粗提物固体得率分别为索氏提取器法2.37%、加热回流法3.04%、超声波提取法2.89%;3种不同溶剂的SDG粗提物固体得率分别为甲醇2.76%、乙醇3.04%、丙酮3.86%;在加热回流条件下,用50%的丙酮溶液提取的SDG得率最高,为5.33%;最佳提取时间为2 h;提取过程中的碱解方法采用Ca(OH)2-H3PO4系统,SDG粗提物纯度为32.48%。结论建立的亚麻籽木酚素提取方法比原方法有所改进,提取时间有所缩短。
Objective To establish a laboratory extraction and detection method of flax lignan-ring-opened Larch phenol diglucoside (SDG). Methods The single factor experiment was carried out for each step of the extraction process during SDG extraction. The effects of different extraction methods, solution, concentration, time and alkali hydrolysis method on the extraction rate of flaxseed SDG were observed. The SDG content. Results The solid yields of SDG crude extracts of three different extraction methods were respectively 2.37% by Soxhlet extractor, 3.04% by heating and refluxing method and 2.89% by ultrasonic extraction. The solid yields of SDG crude extracts of three different solvents were 2.76% of methanol, 3.04% of ethanol and 3.86% of acetone. Under the condition of heating and refluxing, the yield of SDG extracted by 50% acetone solution was the highest, which was 5.33%. The optimal extraction time was 2 h. Using Ca (OH) 2-H3PO4 system, SDG crude extract purity of 32.48%. Conclusion The establishment of flaxseed lignan extraction method improved than the original method, extraction time has been shortened.