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目的:观察环氧合酶-2(COX-2)抑制剂塞来昔布对直肠癌HCA-7细胞株的放射敏感性及探讨其机制。方法:采用MTT法检测塞来昔布作用不同时间对直肠癌HCA-7细胞株增殖的影响,计算出塞来昔布的半数抑制浓度IC50;HCA-7细胞克隆形成实验用于检测塞来昔布对HCA-7细胞的放射敏感性,并绘制存活曲线;流式细胞仪(FCM)测定塞来昔布对HCA-7的细胞周期的影响。结果:塞来昔布对HCA-7细胞株的抑制率随时间的延长而升高,48h的IC50是40.19μmol/L;照射组+药物组的SF2、D0、Dq、SER较单纯照射组均有所下降。塞来昔布使HCA-7细胞发生G2和M期阻滞,并抑制S期的比例。结论:塞来昔布能增加直肠癌HCA-7细胞的放射敏感性。
OBJECTIVE: To observe the radiosensitivity of cyclooxygenase-2 (COX-2) inhibitor celecoxib to rectal cancer HCA-7 cell lines and to explore its mechanism. Methods: The effect of celecoxib on the proliferation of rectal cancer HCA-7 cell line was detected by MTT assay, and the IC50 of celecoxib was calculated. The colony formation assay of HCA-7 cells was used to detect the expression of celecoxib The radiosensitivity to HCA-7 cells was determined and the survival curve was drawn. The effect of celecoxib on the cell cycle of HCA-7 was determined by flow cytometry (FCM). Results: The inhibitory rate of celecoxib on HCA-7 cell line increased with time, and the IC50 at 48h was 40.19μmol / L. The SF2, D0, Dq and SER in the irradiated group and the drug-treated group were significantly higher than those in the irradiated group Decline. Celecoxib caused G2 and M arrest in HCA-7 cells and inhibited the proportion of S phase. Conclusion: Celecoxib increases the radiosensitivity of rectal cancer HCA-7 cells.