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以全基因组测序已经完成的材料粳稻日本晴和完成了第4染色体全序列测序的籼稻广陆矮4号的杂交F2作为构图群体,共90个单株,构建了一张含148个微卫星标记的水稻分子遗传图谱。该F2群体显著偏分离非常高,发现有49个分子标记表现偏分离(P<0.05),占总标记数的33.11%,这些偏分离标记中有36个偏向广陆4号,13个偏向杂合体,没有偏向日本晴的偏分离标记。讨论了配子体基因和孢子体基因导致偏分离的原因,通过已经定位的配子体基因和杂种不育基因分布在偏分离集中的区域来进一步说明配子体基因和杂种不育基因确实是导致偏分离形成的原因,而且还通过未定位的标记分析了偏分离的原因。
A total of 90 plants were crossed with japonica rice Nipponbare, which had been completed by whole genome sequencing, and F2 of indica rice Guangluai 4, which had completed the sequencing of chromosome 4, and a cDNA clone containing 148 microsatellite markers Rice molecular genetic map. The F2 population was significantly segregated to a very high level, and 49 molecular markers were found to segregate (P <0.05), accounting for 33.11% of the total number of markers. Thirty-six of these markers were located in Guanglu 4 and 13 biased Fit, there is no bias Nipponbare partial separation mark. The reasons for the partial segregation of gametophyte gene and sporophyte gene were discussed. The reason why the gametophyte gene and the hybrid sterility gene are further segregated is explained by the distribution of the already-located gametophyte gene and the hybrid sterility gene in the area of partial segregation But also analyzed the reason of partial segregation by undefined markers.