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目的：研究缺氧对人脐静脉内皮细胞珠ＥＣ－３０４ＴＸＳ基因的影响及ＥＰＯ３′增强子在其中的作用。方法：将红细胞生成素（ＥＰＯ）３′－增强子野生片断及点突变片断，借脂质体转入人脐静脉内皮细胞株ＥＣ－３０４，用半定量ＲＴ－ＰＣＲ测定常氧与缺氧条件下培养６小时的细胞血栓素合酶（ＴＸＳ）ｍＲＮＡ。结果：ＥＣ－３０４常氧培养有ＴＸＳ基因表达缺氧６小时ＴＸＳ基因表达明显增强；而细胞导入野生ＥＰＯ３′－增强子片断可阻断缺氧诱导ＴＸＳ基因表达增强，而点突变片断无此作用。结论：在ＴＸＳ基因序列中，可能存在类似ＥＰＯ３′－增强子片断，缺氧诱导ＴＸＳ基因表达增强可能主要通过缺氧诱导因子－１与ＴＸＳ基因序列中类似ＥＰＯ３′－增强子片断结合。
Objective: To investigate the effect of hypoxia on EC-304TXS gene in human umbilical vein endothelial cells and the role of EPO3 ’enhancer in it. Methods: The wild-type and point mutation fragments of erythropoietin (EPO) 3’-enhancer were transfected into human umbilical vein endothelial cell line EC-304 by semi-quantitative RT-PCR. The effects of normoxia and hypoxia 6 hours of cell thromboxane synthase (TXS) mRNA. Results: The expression of TXS gene in EC-304 cells cultured in hypoxia for 6 hours was significantly increased in hypoxia for 6 hours, while the introduction of wild-type EPO3’-enhancer fragment blocked the hypoxia-induced enhancement of TXS gene expression, while the point mutation fragment did not. . CONCLUSION: In the sequence of TXS, EPO3’-enhancer fragment may exist. Hypoxia-induced increase of TXS gene expression may be mainly through hypoxia-inducible factor-1 and similar EPO3’-enhancer fragment in TXS gene sequence.