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以全长人ＴＰＯｃＤＮＡ为模板，采用ＰＣＲ方法扩增出ＴＰＯ功能区段基因，克隆入ＰＵＣ１８中，经筛选鉴定后，插入原核表达载体中进行表达，结果ｒｈＴＰＯ功能区片段在原核细胞中获得高效表达，表达量约占菌体总蛋白的４１５％，表达产物经初步纯化后测活，具有明显促进体外培养巨核细胞集落形成的作用。
The full-length human TPO cDNA was used as a template to amplify the functional fragment of TPO gene by PCR and cloned into PUC18. After screening and identification, the TPO cDNA was inserted into the prokaryotic expression vector for expression. The results showed that the rhTPO functional domain fragment was highly expressed in prokaryotic cells , Which accounted for 415% of the total bacterial protein. The expression product was measured after preliminary purification, which could significantly promote the formation of megakaryocyte colonies in vitro.