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目的:探讨苦参素对免疫性肝纤维化大鼠基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-1抑制剂(TIMP-1)表达的影响。方法:建立猪血清诱导的大鼠免疫性肝纤维化模型。SD雄性大鼠44只,随机分为正常对照组、模型组、秋水仙碱组(0.1 mg.kg-1)和苦参素组(60 mg.kg-1),ip,1次/d,连续8周。采用免疫组化方法检测MMP-1,TIMP-1在各组大鼠中的表达。结果:与模型组相比,苦参素组MMP-1表达为(3.42±0.53)%,高于模型组的(2.81±0.53)%,(P<0.05);苦参素组TIMP-1表达水平为(30.62±3.18)%,明显低于模型组的(41.64±4.51)%,(P<0.01);苦参素组TIMP-1/MMP-1比值也明显低于模型组(P<0.01)。结论:苦参素能增加免疫性肝纤维化大鼠肝脏组织MMP-1的表达,抑制TIMP-1表达,调节TIMP-1/MMP-1比值,通过对细胞外基质(ECM)的调节起到抗肝纤维化的作用。
Objective: To investigate the effect of oxymatrine on the expression of matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-1 inhibitor (TIMP-1) in rats with autoimmune liver fibrosis. Methods: To establish a model of rat immunological liver fibrosis induced by pig serum. Forty-four SD male rats were randomly divided into normal control group, model group, colchicine group (0.1 mg.kg-1) and oxymatrine group (60 mg.kg-1) For 8 weeks. Immunohistochemistry was used to detect the expression of MMP-1 and TIMP-1 in each group. Results: Compared with model group, the expression of MMP-1 in matrine group was (3.42 ± 0.53)%, higher than that in model group (2.81 ± 0.53)%, (P <0.05) (P <0.01). The ratio of TIMP-1 / MMP-1 in matrine group was significantly lower than that in model group (P <0.01) ). Conclusion: Oxymatrine can increase the expression of MMP-1, inhibit the expression of TIMP-1 and regulate the ratio of TIMP-1 / MMP-1 in the liver of immune-induced hepatic fibrosis in rats, and regulate the extracellular matrix (ECM) Anti-fibrosis effect.