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在pH 6.80的NaH2PO4-Na2HPO4缓冲介质中,酸性茜素蓝B(AABB)与阿昔洛韦(ACV)相互作用,形成离子缔合物,溶液颜色发生明显改变,最大褪色波长为526 nm,同时在618 nm波长处产生吸收峰。在最大褪色波长526 nm处,ACV的浓度在1.50×10-6~2.38×10-5mol/L内遵守比尔定律,表观摩尔吸光系数为1.26×104L/(mol.cm),检出限为8.85×10-7mol/L,以此建立测定阿昔洛韦分光光度法。该法具有较高的灵敏度和良好的选择性,用于临床用药、血浆及尿液中阿昔洛韦的测定。
Acidic alizarin blue B (AABB) interacts with acyclovir (ACV) to form ionophores in pH 6.80 NaH2PO4-Na2HPO4 buffer medium. The color of the solution changes significantly with a maximum fading wavelength of 526 nm. At the same time, Absorption peak at 618 nm. Beer ’s law was obeyed at a maximum fading wavelength of 526 nm with an ACV concentration of 1.50 × 10-6 ~ 2.38 × 10-5 mol / L. The apparent molar absorptivity was 1.26 × 104 L / (mol.cm). The detection limit was 8.85 × 10-7mol / L, in order to establish the determination of acyclovir spectrophotometry. The method has high sensitivity and good selectivity for clinical use, determination of acyclovir in plasma and urine.