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目的 :探讨肾细胞癌中FHIT基因的改变及意义。方法 :用RT PCR及Southern印迹杂交法检测肾细胞癌及其细胞系中FHIT基因转录本 ,并观察其基因组的改变。结果 :约 8 3% (2 / 2 4)肾细胞癌有异常转录本 ;其中一株细胞系cDNA在用外引物扩增时 ,只有一个变小的FHIT基因异常转录本 ;用内引物扩增时 ,FHIT基因转录本完全缺失。约 2 0 % (5 / 2 4)肾细胞癌FHIT基因区域的基因组DNA出现重排。结论 :部分肾细胞癌存在FHIT基因转录本及基因组异常 ;FHIT基因的改变在肾细胞癌的发生、发展中可能起一定作用。
Objective: To investigate the changes of FHIT gene in renal cell carcinoma and its significance. Methods: Transcripts of FHIT gene in renal cell carcinoma and its cell lines were detected by RT PCR and Southern blot hybridization, and the changes of its genome were observed. RESULTS: About 8.3% (2/24) of renal cell carcinoma had abnormal transcripts. One of the cell lines had only one smaller transcript of the FHIT gene amplified by using an external primer. Amplification with an internal primer When FHIT gene transcripts were completely deleted. Approximately 20% (5/24) of renal cell carcinoma FHIT gene region rearrangement of genomic DNA. Conclusion: FHIT gene transcripts and genomic abnormalities exist in some renal cell carcinomas. The changes of FHIT gene may play a role in the occurrence and development of renal cell carcinoma.