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为了解卡拉OK话筒污染状况,于1996年12月对全县18家饭店、宾馆的47个卡拉OK话筒进行了卫生学调查,报告如下:1 材料与方法1.1 调查对象 对全县18家饭店、宾馆的47个话筒进行采样检测.1.2 采样及检验方法 检测HBsAg,用棉拭子蘸1%血清磷酸盐缓冲液(0.01mol/L,pH7.2)对所检话筒来回涂抹10次,放入无菌管中,带回实验室,放4℃冰箱过夜,用酶联免疫吸附试验进行检验.大肠菌群使用专用的大肠菌群快速检验纸片进行检验,按说明书进行采样、检验.金黄色葡萄球菌、溶血性链球菌检测,用无菌操作法将浸有生理盐水的棉拭子在话筒上来回涂抹10次,放入无菌管中,经增菌后,用常规法检验.
In order to understand the situation of karaoke microphone pollution, in December 1996 on the county’s 18 restaurants, hotels, 47 karaoke OK microphone hygiene survey, the report is as follows: 1 Materials and Methods 1.1 Survey The county 18 restaurants, Forty-seven microphones of the hotel were sampled and tested.1.2 Sampling and testing methods HBsAg was detected, and a cotton swab dipped in 1% serum phosphate buffer (0.01 mol / L, pH7.2) Sterile tube, back to the laboratory, put the refrigerator at 4 ℃ overnight, with enzyme-linked immunosorbent assay for testing. Coliform bacteria using special coliform rapid test paper, according to the instructions for sampling, testing. Staphylococcus aureus, hemolytic streptococcus detection, asepsis method will be immersed in saline cotton swab back and forth on the microphone smear 10 times, into the sterile tube, the bacteria, by conventional testing.