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目的:研究雌激素对缺血性脑损害的神经保护作用是否与抗神经元凋亡有关。方法:30只雌性成年SD大鼠,随机分为假手术组(sham),手术对照组(OVX),替勃龙组[0.25mg/(kg.d)];采用大鼠大脑中动脉闭塞制成局灶性脑缺血模型。在缺血2h、再灌注24h后立即断头取脑,应用2,3,5-三苯基氯化四唑(TTC)染色观察脑梗死体积,TUNEL法检测凋亡细胞,链酶菌抗生物素蛋白过氧化物酶(SP)法检测抗凋亡基因Bcl-2、细胞凋亡诱导因子Bax和半胱氨酰天冬氨酸特异性蛋白酶caspase-3的表达。结果:替勃龙用药组较手术对照组脑梗死体积显著缩小(P<0.05),TUNEL阳性细胞数减少(P<0.01),Bcl-2阳性细胞增多(P<0.01),Bax及caspase-3阳性细胞减少(P<0.01)。结论:替勃龙可抑制去卵巢大鼠局灶性脑缺血再灌注时神经细胞凋亡,具有神经保护作用。
AIM: To investigate whether the neuroprotective effect of estrogen on ischemic brain damage is related to anti-neuronal apoptosis. Methods: Thirty female adult Sprague-Dawley rats were randomly divided into sham operation group (OVX) and tibolone group (0.25 mg / (kg · d)]. The middle cerebral artery occlusion Into the focal cerebral ischemia model. The brain was decapitated immediately after ischemia for 2 hours and then reperfusion for 24 hours. The volume of cerebral infarction was observed by TTC staining with 2,3,5-triphenyltetrazolium chloride (TTC) staining. The apoptotic cells were detected by TUNEL assay. The expression of anti-apoptotic gene Bcl-2, apoptosis-inducing factor Bax and cysteinyl aspartate specific protease caspase-3 were detected by enzyme-linked immunosorbent assay (ELISA) Results: The volume of TUNEL-positive cells was significantly decreased (P <0.01), the number of Bcl-2 positive cells was increased (P <0.01), Bax and caspase-3 The number of positive cells decreased (P <0.01). Conclusion: Tibolone can inhibit neuronal apoptosis during focal cerebral ischemia-reperfusion in ovariectomized rats and has neuroprotective effect.