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为了研究茄子空间诱变效果,比较空间诱变系与原始对照间的分子水平差异,揭示空间诱变的分子效应,对3个不同茄子原始自交系(K_1、L_1、M_1)及其对应空间诱变系(K_2、L_2、M_2)生物学性状进行了比较研究,并利用AFLP分子标记技术进行了DNA指纹分析。结果表明,与原始自交系相比,3个空间诱变系的生育期、株型、果形均未发生明显变化,发生变化的性状是平均单果重和种子千粒重。诱变系M_2的平均单果重比其对照增加16.82%,差异极显著;种子千粒重有降低趋势,其中L_2比L_2显著降低了10.24%。48对AFLP选择性扩增引物在6个自交系和诱变系间扩增得到40条可以揭示诱变系与原始自交系间多态性的条带;部分目标片段测序结果经BLAST分析表明与已报道的高频突变相关DNA区域或蛋白同源性很高。可见,空间诱变技术能够引起茄子的遗传变异,而且这种变异是以DNA水平的变异为基础的。
In order to study the effect of space mutagenesis in eggplant, the molecular effects of space mutagenesis were compared by comparing the molecular level differences between space mutagenesis lines and the original control. The effects of space mutagenesis on three different eggplant inbred lines (K_1, L_1, M_1) The biological characters of mutagenesis lines (K_2, L_2, M_2) were compared and the DNA fingerprinting was analyzed by AFLP molecular marker. The results showed that compared with the original inbred lines, no significant changes were observed in the growth stages, plant type and fruit shape of the three space-induced mutants, and the average trait weight and seed weight per plant were unchanged. The average single fruit weight of M_2 increased by 16.82% compared with that of the control. The difference was significant. The 1000-grain weight of the mutagenesis line tended to decrease, and L_2 was significantly decreased by 10.24% compared with L_2. 48 AFLP amplified primers were used to amplify 40 AFLP-amplified bands between 6 inbred lines and mutagenic lines to reveal the polymorphism of the mutagenesis lines and the original inbred lines. Some of the target fragments were sequenced by BLAST It showed high homology with the DNA region or protein related to the reported high frequency mutation. Visible, spatial mutagenesis technology can cause eggplant genetic variation, and this variation is based on the variation of DNA level.