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目的:建立反相离子对色谱法测定复方维生素片中维生素B1、维生素B2、维生素B6含量的方法。方法:以己烷磺酸钠作为离子对试剂,采用Diamonsil C18(250 mm×4.6 mm,5μm)柱,同时测定复方维生素片中维生素B1、B2和B6的含量。结果:维生素B1、B2和B6分别在6.3～14.7μg.mL-1(r=0.999 8),7.2～16.8μg.mL-1(r=0.999 5),6.3～14.7μg.mL-1(r=0.999 6)范围内线性关系良好,平均回收率分别为100.08%(RSD=0.84%,n=9),100.35%(RSD=1.26%,n=9),99.41%(RSD=1.10%,n=9)。结论:本方法快速、简便、准确,分离效果好,辅料无干扰,可用于该类制剂中维生素B1,B2和B6的含量测定及质量控制研究。
Objective: To establish a method for the determination of vitamin B1, vitamin B2 and vitamin B6 in compound vitamin tablets by reversed-phase ion pair chromatography. Methods: Diamonsil C18 (250 mm × 4.6 mm, 5 μm) column was used with sodium hexanesulfonate as ion-pair reagent to determine the content of vitamin B1, B2 and B6 in compound vitamin tablets simultaneously. Results: Vitamin B1, B2 and B6 were significantly higher in the range of 6.3-14.7μg.mL-1 (r = 0.999 8), 7.2-16.8μg.mL-1 (r = 0.999 5) = 0.999 6). The average recoveries were 100.08% (RSD = 0.84%, n = 9), 100.35% (RSD = 1.26%, n = 9) and 99.41% = 9). Conclusion: The method is rapid, simple and accurate, the separation effect is good, the excipients have no interference, and can be used for the determination of vitamin B1, B2 and B6 and quality control of these preparations.