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应用免疫荧光方法（ＩＦＡ）对湖北省东北部地区的英山县进行了莱姆病的流行病学调查。结果，人群总感染率为８．８％（２４／２７２），其中林区居民及非林区居民感染率分别为１２．８％（２２／１７２）及２．０％（２／１００），两者差异有高度显著性（Ｐ＝０．００５）；共采集蜱４８４只，其中微小牛蜱３０８只，豪猪血蜱１３６只，二棘血蜱４０只。经检测，不同蜱种带菌率为０～１５％。本调查证实湖北省东北部地区存在莱姆病，人群感染菜姆病与环境性蜱暴露有密切关系。
The immunofluorescence method (IFA) was used to investigate the epidemiology of Lyme disease in Yingshan County, northeast of Hubei Province. Results The overall infection rate was 8.8% (24/272). Among them, the infection rates among residents in non-forest area and non-forest area were 12.8% (22/172) and 2.0% (2/100) respectively, The difference was highly significant (P = 0.005). A total of 484 ticks were collected, of which 308 were M. bovis, 136 porcupine hedgehog and 40 B. spinosus. After testing, different tick species carrying rate of 0 to 15%. This survey confirmed the presence of Lyme disease in northeastern Hubei Province. Infection with Lemosis and environmental ticks was closely related to the population.