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AIM: To study the effects of annexins II and V on the survival and neurite outgrowth of primary cultured neuronsand the survival of astrocytes after peroxide and hypoxia insults in vitro. METHODS: Annexins II and V proteinsand/or corresponding antibodies were added to the medium of primary neocortical cultures. H2O2 and NaN3 wereused to induce neuron injury, respectively. Lactate dehydrogenase (LDH) release was measured. RESULTS:Addition of annexin II or V into the culture medium did not affect the normal survival and neurite outgrowth ofcortical neurons. However, when an antibody against annexin II or V was added to the culture, the survival andneurite outgrowth of these neurons markedly declined. Further, addition of the two annexins into cortical culturesafter peroxide and hypoxia insults markedly reduced the LDH release and cell death. CONCLUSION: Annexins IIand V are essential for the survival and neurite outgrowth of developing cortical neurons, the survival of glial cells,and protect neurons and glial cells against peroxide and hypoxia injuries.
AIM: To study the effects of annexins II and V on the survival and neurite outgrowth of primary cultured neurons and the survival of astrocytes after peroxide and hypoxia insults in vitro. METHODS: Annexins II and V proteins and / or corresponding antibodies were added to the medium of RESULTS: Addition of annexin II or V into the culture medium did not affect the normal survival and neurite outgrowth of cortical neurons. However, Further, the addition of the two annexins into cortical cultures after peroxide and hypoxia insults markedly reduced the LDH release and cell death. CONCLUSION: Annexins II and V are essential for the survival and neurite outgrowth of developing cortical neurons, the survival of glial cells , and protect neurons and glial cells against peroxide and hypoxia injuries.