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从感染动物中取得成虫,消化动物的肌肉获得幼虫,分别经洗涤、固定、包埋制成半薄或超薄切片,干燥后用于免疫荧光和免疫标记试验。所用的单克隆抗体分别为NIMM,(免疫沉淀105、90、57和47KDa肌肉期幼虫表膜抗原),NIM-M_2(免疫沉淀90和47KDa肌肉期幼虫表膜抗原),NIM-M_3、NIMM_4和NIM-M_7(免疫沉淀40和20KDa成虫表膜抗原)。将半薄切片加入单克隆培养上清或1:50、1:100稀释的腹水进行孵育,再加荧光标记的抗鼠Ig进行免疫荧光试验,试
Obtained from infected animals, adult animals were digested, and larvae were obtained from the muscles of the animals. The larvae were washed, fixed and embedded to give semi-thin or ultra-thin sections, respectively. After drying, they were used for immunofluorescence and immunolabeling assays. The monoclonal antibodies used were NIMM (immunoprecipitated 105, 90, 57 and 47 kDa muscular larval surface antigens), NIM-M_2 (immunoprecipitated 90 and 47 kDa muscular larval surface antigens), NIM-M 3, NIMM 4 and NIM-M_7 (immunoprecipitated 40 and 20 kDa adult surface antigens). Semi-thin sections were added to the monoclonal culture supernatant or 1: 50, 1: 100 dilution of ascites incubated, coupled with fluorescent-labeled anti-mouse Ig immunofluorescence test, the test