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为获得较高黄酮提取率,采用响应面法优化复合酶提取工艺条件。通过对提取时间、酶用量、提取温度、pH、料液比5个影响因素进行单因素试验基础上,以黄酮提取率为响应值,采用Box-Behnken中心组合设计方法,建立回归模型。利用软件Desing Expert V8.0.6作响应面分析,得出理论最佳工艺参数。对所得理论参数调整后,得到最优工艺参数为:提取温度55.0℃,pH5.5,料液比1:60g/mL,酶用量100μL,提取时间150 min,在该条件下黄酮类化合物提取率为(35.207±1.56)mg/g。通过验证试验可知,建立的数学模型能够较好地预测试验结果。
In order to obtain higher extraction rate of flavonoids, response surface methodology was used to optimize the extraction conditions of the complex enzyme. Based on the single-factor experiments of five influencing factors, such as extraction time, enzyme dosage, extraction temperature, pH and solid-liquid ratio, a regression model was established by using the Box-Behnken central combination design with the flavonoid extraction rate as the response value. Using software Desing Expert V8.0.6 for response surface analysis, the theoretical optimum process parameters were obtained. After the theoretical parameters were adjusted, the optimum technological parameters were as follows: extraction temperature 55.0 ℃, pH 5.5, solid-liquid ratio 1: 60g / mL, enzyme dosage 100μL, extraction time 150 min, extraction conditions of flavonoids (35.207 ± 1.56) mg / g. Through the verification test shows that the established mathematical model can better predict the test results.