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恙虫病的病原体检查方法,以往国内外均用动物(小白鼠等)接种分离,设备较难,操作繁多,需时10～16日。基层单位多无此项设备,但病原体检查,在临床诊断,流行病学调查上均是重要依据。三年来,我们试用病人之焦痂溃疡物或肿大的淋巴结中心穿刺液,发热期血液三种直接标本分别涂片,马氏染色法(Maechiav—ello’s)。在油镜下,查见呈蓝色,双球形或球秆状,较细菌小,在胞浆内或外的立克次体。选择了发病10日内,发热期,未用抗生素或用药不到48小时,有皮肤焦痂溃疡的病人80例作溃疡内容物涂片查见立克次体56例,阳性率占70％。同时抽血作外斐氏OX_K凝集试验,阳性(1∶80以上)46例(占51％)。淋巴结中心穿刺液涂片16例中13例查
Tsutsugamushi disease pathogen check method, both at home and abroad with animals (mice, etc.) vaccination separation, equipment more difficult to operate a variety of takes 10-16 days. More grassroots units without this equipment, but the pathogen examination, clinical diagnosis, epidemiological investigations are important basis. In the past three years, we tested patients with eschar or ulcer or enlarged lymph node center puncture fluid, fever, blood samples of the three direct smear, Markov staining (Maechiav-ello’s). In the oil mirror, check the blue, double spherical or ball-like, more bacteria, in the cytoplasm or outside of Rickettsia. Choose the onset of 10 days, the fever period, no antibiotics or medication less than 48 hours, 80 patients with skin eschar ulcer ulcer content smear check rickettsia 56 cases, the positive rate of 70%. At the same time, blood samples were collected for Oxi_K agglutination test, 46 cases (51%) were positive (1:80 or above). Lymph node puncture fluid smear in 16 cases in 13 cases investigation