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为寻求丙型肝炎病毒基因免疫的最佳动物实验方法，探讨不同处理方式对重组体ｐＣＤ－ＨＣＶ１诱发Ｂａｌｂ／ｃ小鼠产生抗体的影响。采用分子生物学技术构建了ＨＣＶ重组体，用不同免疫次数、途经、剂量和不同处理方式将其免疫小鼠。结果显示：重组体ｐＣＤ－ＨＣＶ１（１００μｇ／只／次，ｎ＝１２）经肌肉１次、２次、３次和４次免疫小鼠后，抗体水平分别为０．１８３±０．０６，０．４２８±０．０５，０．７０７±０．０８（ＯＤ４１０值，下同），其中４次免疫组抗体水平最高；小鼠经灌胃、腹腔注射、皮下注射和肌肉注射（１００μｇ／只×３次）不同途径分别免疫小鼠（ｎ＝６），抗体水平依次为０．１３８±０．０５，０．１７８±０．０７，０．２３３±０．０８和０．６９１±０．０５；以不同剂量（１０μｇ，５０μｇ，１００μｇ）分别免疫鼠（ｎ＝８），抗体水平依次为０．１１０±０．０９；０．３３０±０．０４和０．７００±０．０７，各组间差异均有显著性意义（Ｐ＜０．０１）；用普鲁卡因１００μｌ（０．０４ｍｇ）肌肉注射２４ｈ后，再肌肉、皮下注射ｐＣＤ－ＨＣＶ１，抗体水平比直接肌肉、皮下注射同剂量重组体明显增高。该研究结果为寻求ＨＣＶ－ＤＮＡ疫苗动物实验最佳免疫?
In order to find out the best animal experiment method for hepatitis C virus gene immunization, the effects of different treatment methods on antibody production in Balb / c mice induced by recombinant pCD-HCV1 were explored. Recombinant HCV was constructed by molecular biology techniques and immunized mice with different times of immunization, route of administration, dosage and different treatments. The results showed that the antibody levels of mice immunized once, twice, three times and four times with recombinant pCD-HCV1 (100μg / mouse / time, n = 12) were 0.183 ± 0.06,0 .428 ± 0.05,0.707 ± 0.08 (OD410 value, the same below), among which the antibody level in the four immunized groups was the highest; the mice were intragastrically, intraperitoneally, subcutaneously, and intramuscularly injected 3 times) were immunized by different ways (n = 6), the antibody levels followed by 0.138 ± 0.05,0.178 ± 0.07,0.233 ± 0.08 and 0.691 ± 0.05 (N = 8) were immunized with different doses (10μg, 50μg, 100μg). The antibody levels were 0.110 ± 0.09; 0.330 ± 0.04 and 0.700 ± 0.07, (P <0.01). After intramuscular injection of procaine 100μl (0.04mg) for 24h, Meat, subcutaneous injection of pCD-HCV1, antibody levels were significantly higher than the direct muscle, subcutaneous injection of the same dose of recombinant. The results of this study seek the best immune for HCV-DNA vaccine animal experiments?