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在先前的研究中通过抑制缩减杂交获得了一个在巴西橡胶树胶乳中特异表达的片段(HbSSH10),该片段含有“RINGfinger”或“C3HC4”保守序列。根据HbSSH10的序列信息设计引物并通过3’-RACE和5’-RACE的方法,获得了一个全长的cDNA(HbRZF)。该cDNA含有589个核苷酸,含有完整的阅读框架,编码156个氨基酸。从它推导出的氨基酸序列中含有“RINGfinger”或“C3HC4”保守区(氨基酸100￣144)。该氨基酸序列与Poncirustrifoliata、Arabidopsisthaliana和Thellungiellahalophila的环锌指蛋白的同源性分别为48%、52%和50%。Northern杂交分析表明HbRZF在胶乳中大量表达,在叶片中微量表达,而在根和花中几乎没有表达。茉莉酸处理可以诱导胶乳中HbRZF的表达,而乙烯对胶乳中HbRZF的表达基本上没有影响。
In a previous study, a fragment (HbSSH10) specifically expressed in rubber gum latex was obtained by suppression subtractive hybridization and contained “RINGfinger” or “C3HC4” conserved sequences. According to the sequence information of HbSSH10, primers were designed and a full-length cDNA (HbRZF) was obtained by 3’-RACE and 5’-RACE. The cDNA contains 589 nucleotides and contains a complete reading frame encoding 156 amino acids. The deduced amino acid sequence contains “RINGfinger” or “C3HC4” conserved regions (amino acids 100-144). This amino acid sequence shares 48%, 52% and 50% homology with the loop zinc finger protein of Poncirus trifoliata, Arabidopsisthaliana and Thellungiellahalophila, respectively. Northern blot analysis showed that HbRZF was abundantly expressed in latex and slightly expressed in leaves, with little expression in roots and flowers. JA treatment induced the expression of HbRZF in latex, while ethylene had no effect on the expression of HbRZF in latex.