论文部分内容阅读
从体表具有明显出血症状的患病泥鳅内脏组织中分离到2株优势菌,对这2种菌进行了鉴定和特性分析。利用形态学观察、生理生化特性分析和16SrDNA序列测定法对细菌类别进行初步判定,并采用人工感染试验和药敏试验对细菌的致病性与耐药性进行分析。分析表明这2种菌分别为霍乱弧菌(Vibrio cholerae)和希瓦氏菌(Shewanella putrefaciens),人工感染试验表明它们对泥鳅具有较强的致病性;用含有抗生素的20种药敏纸片进行药敏试验,发现这2种分离菌同时对氯霉素、环丙沙星、复方新诺明、头孢西丁、四环素、诺氟沙星和头孢他啶等7种抗生素高敏,对其他抗生素具有一定抗性。首次报道了泥鳅一种新的病原即希瓦氏菌,试验表明希瓦氏菌作为一种潜在的病原菌,可能与泥鳅霍乱弧菌协同作用,对泥鳅的养殖造成危害,同时也为泥鳅常见体表出血症病原的确定和防治提供参考依据。
Two predominant bacteria were isolated from visceral tissues of diseased Loach loach which had obvious bleeding on the body surface. The two kinds of bacteria were identified and characterized. The bacterial species were preliminarily determined by morphological observation, physiological and biochemical characteristics and 16S rDNA sequencing. The pathogenicity and drug resistance of the bacteria were analyzed by artificial infection test and susceptibility test. Analysis showed that these two kinds of bacteria were Vibrio cholerae and Shewanella putrefaciens, respectively, and artificial infection tests showed that they were highly pathogenic to loach. The antibiotic-resistant 20 kinds of drug-susceptible paper According to the drug susceptibility test, it was found that the two isolates were highly sensitive to 7 antibiotics such as chloramphenicol, ciprofloxacin, cotrimoxazole, cefoxitin, tetracycline, norfloxacin and ceftazidime, and had certain resistance to other antibiotics Sex. The first report of a new pathogen of loach that Shewanella, the test showed that Shewanella as a potential pathogen, may act synergistically with loach Vibrio cholerae, causing harm to the cultivation of loach, but also common loach Table bleeding pathogen identification and prevention provide the reference.