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目的探讨微生物培养标本不合格的原因及对策。方法选取徐州仁慈医院2014年3月—2015年11月收集的4547份微生物培养标本,其中970份为不合格标本。回顾性分析标本的临床检验结果,并对导致微生物培养标本临床检验不合格的原因进行探讨,并提出针对性的解决对策。结果微生物培养标本不合格率为21.33%(970/4547),其中,创伤分泌物标本占61.86%(600/970),痰液标本占20.62%(200/970),血液标本占10.31%(100/970),大便标本占5.15%(50/970),其他标本占2.06%(20/970)。造成微生物培养标本不合格的因素主要包括凝血、药品器械消毒不彻底、溶血、人员操作不当、痰液标本多为唾液、采血量少、试管错误、乳糜血、粪便标本少且不新鲜、采血容器不当、胸腹腔积液凝固及其他原因。结论合格的微生物培养标本是提高临床检验成功率的重要指标,检验科医护人员要建立预防对策,降低微生物培养标本的不合格率,提高检验科的工作效率,为临床诊断和治疗提供重要依据。
Objective To investigate the causes of unqualified microbial culture specimens and its countermeasures. Methods 4547 microbial culture samples collected from March 2014 to November 2015 in Renzhou Hospital of Xuzhou were selected, of which 970 were unqualified. Retrospective analysis of specimens of clinical test results, and lead to microbial culture specimens of the reasons for the failure of clinical testing to explore and propose targeted solutions. Results The percent of unqualified samples was 21.33% (970/4547), of which 61.86% (600/970) were specimens of wound exudates, 20.62% (200/970) of sputum samples and 10.31% (100) of blood samples / 970), stool specimens accounted for 5.15% (50/970), other specimens accounted for 2.06% (20/970). The factors leading to the failure of microbial culture specimens include coagulation, incomplete disinfection of drug equipment, hemolysis, improper personnel handling, sputum specimens mostly saliva, less blood collection, wrong test tubes, chyle blood, stool specimens and less fresh blood collection containers Improper, pleural effusion solidification and other reasons. Conclusion The qualified microorganism culture samples are important indicators to improve the success rate of clinical examination. Laboratory medical staffs should set up preventive measures, reduce the unqualified rate of microorganism culture samples and improve the work efficiency of laboratory, which provides an important basis for clinical diagnosis and treatment.