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目的 :检测抑癌基因PTEN在鼻咽癌组织的表达缺失情况 ,探讨PTEN与鼻咽癌发生发展之间的关系。方法 :采用免疫组织化学SABC染色法观察鼻咽癌旁正常组织和鼻咽癌组织中PTEN蛋白的表达 ,并分析其与病理组织类型、病理分级和临床分期的关系。结果 :PTEN在鼻咽癌旁正常组织的表达缺失率 (13.3% )低于鼻咽癌组织的表达缺失率 (47.8% ) ,其差异有统计学意义 (P <0 .0 5 ) ;鼻咽鳞状细胞癌PTEN的表达缺失率(5 8.1% )高于分化型和非分化型非角化癌 (30 .8% ) ;高、中、低分化的鼻咽鳞状细胞癌中 ,PTEN的表达缺失率依次递增 (分别为 2 5 .0 %、6 3.2 %、83.3% ) ;鼻咽癌临床Ⅲ～Ⅳ期的PTEN的表达缺失率 (78.1% )高于临床Ⅰ～Ⅱ期 (2 1.6 % )。结论 :PTEN在鼻咽癌的发生及发展过程中有表达缺失 ,且与病理类型、病理分级、临床分期有关。
Objective: To detect the expression of tumor suppressor gene PTEN in nasopharyngeal carcinoma and to explore the relationship between PTEN and the occurrence and development of nasopharyngeal carcinoma. Methods: Immunohistochemical SABC staining was used to detect the expression of PTEN protein in normal and nasopharyngeal tissues adjacent to nasopharyngeal carcinoma. The relationship between the expression of PTEN protein and pathological type, pathological grade and clinical stage was analyzed. Results: The loss of PTEN expression in nasopharyngeal carcinoma was lower than that in nasopharyngeal carcinoma (13.8%) (47.8%), the difference was statistically significant (P <0.05) The loss of PTEN expression in squamous cell carcinoma (5.11%) was higher than that in differentiated and non-differentiated non-keratinizing carcinoma (30.8%). In high, moderate and poorly differentiated nasopharyngeal squamous cell carcinoma, PTEN The deletion rate of PTEN in stage Ⅲ ~ Ⅳ nasopharyngeal carcinoma (78.1%) was higher than that in stage Ⅰ ~ Ⅱ (25.6%, 32.2%, 83.3% respectively) %). Conclusions: The expression of PTEN in nasopharyngeal carcinoma is related to the pathological type, pathological grade and clinical stage.