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植物毒素Gelonin是一种强有力的蛋白合成抑制物,对完整细胞无作用,与适当的载体连接可以对抗恶性肿瘤细胞.目前普遍采用的检测Gelonin的RIA法,存在需使用有害化合物、孵育时间长、需将游离的与结合的Gelonin分开以及既使于4℃保存也可产生明显的聚集等缺点,严重地限制了这一方法的应用.现介绍一种可用于测定Gelonin的简单、快速、均相的脂质体免疫溶解试验(LI-LA).其基本原理是:表面吸附有Gelonin的脂质体与抗-Gelonin
Plant toxin Gelonin is a potent inhibitor of protein synthesis that has no effect on intact cells and is able to fight malignant cells when ligated with an appropriate carrier.Generally used Gelonin RIA methods require the use of harmful compounds and long incubation times , The need to separate free and combined Gelonin and even at 4 ° C preservation can also produce significant aggregation and other shortcomings, severely limited the application of this method is described one can be used to determine Gelonin simple, fast, are Phase liposome immunolysis test (LI-LA) .The basic principle is: the surface of Gelonin liposomes and anti-Gelonin