表没食子儿茶素没食子酸酯诱导HL60细胞程序性死亡

• 作 者: 赵燕 曹进 祝和成 马洪 刘箭卫
• 机 构: 湖南医科大学茶与健康研究室,湖南医科大学肿瘤研究所
• 出 处: 中国药学杂志
• 发表日期: 1998年08期
• 关键词: HL 细胞程序性死亡 透射电镜技术 作用机制 细胞存活率 琼脂糖凝胶电泳 凋亡小体 基因表达 杀伤作用 小鼠皮肤

目的：探讨儿茶素类化合物的抗癌作用机制。方法：以儿茶素的主要成分表没食子儿茶素没食子酸酯（ＥＧＣＧ）为实验材料，使用噻唑蓝（ＭＴＴ）还原法，ＤＮＡ凝胶电泳，透射电镜技术，观察了ＥＧＣＧ对人急性早幼粒白血病细胞（ＨＬ６０）程序性死亡的影响。结果：０．５５×１０－３ｍｏｌ·Ｌ－１ＥＧＣＧ作用５ｈ后，琼脂糖凝胶电泳下，ＨＬ６０细胞呈现典型的ＤＮＡ条带，透射电镜观察到凋亡小体。结论：ＥＧＣＧ对ＨＬ６０细胞程序性死亡的诱导活性平行于它的细胞杀伤作用

Objective: To investigate the anti-cancer mechanism of catechins. Methods: The epicatechin gallate (EGCG), a main component of catechin, was used as the experimental material. MTT assay, DNA gel electrophoresis and transmission electron microscopy were used to observe the effect of EGCG on acute precocious The impact of programmed cell death in leukemic cells (HL-60). Results: After treated with 0.55 × 10-3mol·L-1EGCG for 5h, HL60 cells showed typical DNA bands under agarose gel electrophoresis. Apoptotic bodies were observed by transmission electron microscopy. CONCLUSION: The induction activity of EGCG on HL60 cell apoptosis is parallel to its cell killing effect