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１９９１～１９９３年在湖南省部份地区对孕妇进行了破伤风类毒素免疫，为检查免疫效果，在新田、醴陵和浏阳３县收集了１７０对母亲及其新生儿的血液标本，采用间接血凝试验检测了母亲免疫前、后和新生儿脐带血的破伤风抗毒素，母亲免疫前、后的阳性率分别为８．２４％和９７．０６％，新生儿的阳性率为８７．６５％；抗毒素含量母亲免前免后各为０．００４８ＩＵ／ｍｌ和０．１３７ＩＵ／ｍｌ，新生儿为０．０５４ＩＵ／ｍｌ。未发现异常反应。证明对孕妇进行破类免疫，能保护新生儿免受破伤风感染。
From 1991 to 1993, pregnant women were immunized with tetanus toxoid in some areas of Hunan Province. In order to check the immune effect, blood samples were collected from 170 mothers and their newborns in Xintian, Liling and Liuyang counties. Indirect blood The coagulation test detected tetanus antitoxin before and after maternal immunization and neonatal umbilical cord blood. The positive rates of maternal tetanus toxin before and after immunization were 8.24% and 97.06% respectively, and the positive rate of neonates was 87.65%. Antitoxin-free mothers were 0.0048 IU / ml and 0.137 IU / ml, respectively, and newborns 0.054 IU / ml. No abnormal reaction was found. Demonstrate that immunizations against pregnant women can protect newborns from tetanus.