【摘 要】
：
Beak and feather disease virus (BFDV) is an infectious agent responsible for feather degeneration and beak deformation in birds.In March 2017,an epidemic of psittacine beak and feather disease (PBFD) struck a farm in Fuzhou in the Fujian Province of south
【机 构】
：
Fujian Agriculture and Forestry University, Fuzhou 350002,China;Fuzhou Zoo, Fuzhou 350012, China;CAS
论文部分内容阅读
Beak and feather disease virus (BFDV) is an infectious agent responsible for feather degeneration and beak deformation in birds.In March 2017,an epidemic of psittacine beak and feather disease (PBFD) struck a farm in Fuzhou in the Fujian Province of southeast China,resulting in the death of 51 parrots.In this study,the disease was diagnosed and the pathogen was identified by PCR and whole genome sequencing.A distinct BFDV strain was identified and named as the FZ strain.This BFDV strain caused severe disease symptoms and pathological changes characteristic of typical PBFD in parrots,for example,loss of feathers and deformities of the beak and claws,and severe pathological changes in multiple organs of the infected birds.Phylogenetic analysis showed that the FZ strain was more closely related to the strain circulating in New Caledonia than the strains previously reported in China.Nucleotide homology between the FZ strain and other 43 strains of BFDV ranged from 80.0％ to 92.0％.Blind passage experiment showed that this strain had limited replication capability in SPF Chicken Embryos and DF-1 Cells.Furthermore,the capsid (Cap) gene of this FZ strain was cloned into the pGEX-4T-1 expression vector to prepare the polyclonal anti-Cap antibody.Western blotting analysis using the anti-Cap antibody further confirmed that the diseased parrots were infected with BFDV.In this study,a PBFD and its pathogen was identified for the first time in Fujian Province of China,suggesting that future surveillance of BFDV should be performed.
其他文献
Conventional influenza vaccines need to be designed and manufactured yearly.However,they occasionally provide poor protection owing to antigenic mismatch.Hence,there is an urgent need to develop universal vaccines against influenza virus.Using nucleoprote
Histone deacetylase (HDAC) inhibitors show clinical promise for the treatment of cancers,including hepatocellular carcinoma (HCC).In this study,we investigated the effect of HDAC inhibitor treatment on hepatitis C virus (HCV)replication in Huh7 human live
The World Society for Virology (WSV) was founded and incorporated as a nonprofit organization in the United States in 2017.WSV seeks to strengthen and support both virological research and virologists who conduct research of viruses that affect humans,oth
Dear Editor,rnIn 2003,severe acute respiratory syndrome coronavirus (SARS-CoV) emerged in Guangdong Province,China,infected more than 8000 individuals,and resulted in a 10％ mortality rate (Rota et al.2003).Later,in 2012,a novel CoV,Middle East respiratory
This study aimed to investigate the serological characteristics of Ebola virus (EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone.In total,877 blood samples from 694 suspected Ebola virus disease (EVD) cases assessed from March t
Dear Editor,rnMammalian cells encode five members of the nucleosome assembly protein 1 (NAP1) family of proteins including nucleosome assembly protein 1-like 1 (NAP1L1),NAP1L2,NAP1L3,NAP1L4 and NAP1L5 (Attia et al.2011).NAP1L1 is ubiquitously expressed an
Dear Editor,rnSince December 2019, the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induced human disease, the coronavirus disease 2019 (COVID-19),has been reported in more than 200 countries and areas around the world (Wu et a
Dear EditorrnMarine mammals are widely distributed and can be found almost in all coastal waters and coastlines around the world.The interface areas between marine and terrestrial environments provide natural habitats for aquatic and semiaquatic mammals a
Alzheimer\'s disease (AD),also known as Alzheimer\'s,is a chronic neurodegenerative disorder with hallmark amyloid plaques in brain tissue.The diseases commences slowly and worsens over time (Sjogren et al.1952).Although it has been investigated for o
Porcine reproductive and respiratory syndrome virus (PRRSV) is a member within the family Arteriviridae of the order Nidovirales.Replication of this positive-stranded RNA virus within the host cell involves expression of viral replicase proteins encoded b