论文部分内容阅读
目的:研究普伐他汀在大鼠小肠的吸收情况。方法:采用大鼠在体小肠回流实验装置,利用HPLC紫外检测的方法测定肠循环液中酚红和普伐他汀的含量。采用XTerra@MS C-18色谱柱(5μm,150mm×2.1 mm.ID),流动相为3.5 mmol/L磷酸二氢钠溶液—乙腈(70:30,用磷酸调至pH 3.0),流速为0.2ml/min;结果:普伐他汀在大鼠小肠全肠段的吸收速率常数和吸收百分率分别为0.110±0.023(h~(-1))和18.21±2.50%。普伐他汀在小肠中吸收量与时间呈线性关系,但吸收速率较低。结论:普伐他汀可以通过增加药物的脂溶性,进而提高药物的生物利用度。
Objective: To study the absorption of pravastatin in the small intestine of rats. Methods: Rat intestinal rehydration in vivo experimental apparatus, the use of HPLC UV detection of intestinal circulating fluid phenol red and pravastatin levels. The mobile phase was a 3.5 mmol / L sodium dihydrogen phosphate solution - acetonitrile (70:30, adjusted to pH 3.0 with phosphoric acid) using a XTerra @ MS C-18 column (5 μm, 150 mm × 2.1 mm.ID) ml / min. Results: The rate constant and absorption rate of pravastatin in the intestine of intestine were 0.110 ± 0.023 (h ~ (-1)) and 18.21 ± 2.50%, respectively. Pravastatin absorption in the small intestine and the linear relationship between the time, but the absorption rate is low. Conclusion: Pravastatin can increase the bioavailability of drugs by increasing the lipid-solubility of the drugs.