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真核细胞基因特定的活化机制几乎不知道,但一般认为RNA聚合酶Ⅱ催化的转录作用是基因表达的最基本过程,因而这个酶对RNA合成的调节作用是真核细胞基因表达的一个重要研究课题。本实验从艾氏腹水瘤细胞中纯化了一种蛋白质,即蛋白因子S-Ⅱ,是一种碱性蛋白质,分子量40500,具有刺激RNA聚合酶Ⅱ的活性。用S-Ⅱ制备了抗S-Ⅱ血清,按Marluft法分离艾氏腹水瘤细胞核进行研究。已经知道细胞核中有三种RNA聚合酶(Ⅰ～Ⅲ),聚合酶Ⅰ合成核糖体RNA,聚合酶Ⅲ合成小分子量RNA,这两种酶的活性不受α-鹅膏蕈碱(α-amanitin)的影响。RNA聚合酶Ⅱ合成mRNA,它的活性可被α-aminitin抑制,实验观
The specific activation mechanism of eukaryotic genes is scarcely known. However, RNA polymerase II-catalyzed transcription is considered to be the most basic process of gene expression. Therefore, the regulatory effect of this enzyme on RNA synthesis is an important study of eukaryotic gene expression Question. In this study, purified from Ehrlich ascites tumor cells a protein, the protein factor S-Ⅱ, is a basic protein, molecular weight 40,500, with the stimulation of RNA polymerase Ⅱ activity. S-Ⅱ anti-S-Ⅱ serum was prepared by Marluft method Ehrlich ascites tumor nuclei were studied. It has been known that there are three types of RNA polymerase (I ~ III) in the nucleus, the polymerase I synthesizes ribosomal RNA and the polymerase III synthesizes small molecular weight RNA. The activities of these two enzymes are not affected by α-amanitin, Impact. RNA polymerase II mRNA synthesis, its activity can be α-aminitin inhibition, the experimental concept