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目的探讨单唾液酸神经节苷脂(GM1)对脑缺血再灌注大鼠海马白介素-6(IL-6)表达的影响。方法 72只SD大鼠随机分为:假手术组(Sham,8只)、神经节苷脂治疗组(GM1,32只)、缺血再灌注组(I/R,32只)。GM1治疗组和I/R组依据缺血后再灌注的不同时间点再细分为6、12、24和3 d 4个小组。应用免疫组织化学方法和Western blot方法检测各组大鼠海马IL-6蛋白的表达。结果免疫组织化学方法检测时发现,IL-6在假手术组大鼠的海马即有微量表达,而在脑缺血6 h时表达迅速升高,24 h时表达最高;与相同时间点的缺血组比较,GM1治疗组的IL-6表达则明显降低(P<0.05);Western blot方法也得到了相同的结论。结论GM1很可通过减少IL-6的表达参与脑缺血再灌注损伤的神经保护作用。
Objective To investigate the effect of monosialoganglioside (GM1) on the expression of interleukin-6 (IL-6) in the hippocampus after cerebral ischemia-reperfusion in rats. Methods Seventy-two SD rats were randomly divided into sham-operated group (sham, 8 rats), ganglioside-treated group (GM1, 32) and ischemia-reperfusion group (32 rats). The GM1-treated group and the I / R group were further subdivided into 4 groups at 6, 12, 24 and 3 days according to the different time points after ischemia-reperfusion. Immunohistochemistry and Western blot were used to detect the expression of IL-6 in hippocampus of rats in each group. Results The results of immunohistochemistry showed that IL-6 was slightly expressed in hippocampus of rats in sham-operation group and rapidly increased at 6 h after cerebral ischemia, and was highest at 24 h. Compared with the same time point Compared with the blood group, the expression of IL-6 in GM1 treatment group was significantly decreased (P <0.05). The same conclusion was obtained by Western blot. Conclusion GM1 may play a neuroprotective role in cerebral ischemia-reperfusion injury by decreasing the expression of IL-6.