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目的:研究在核因子κB受体活化因子配基(RANKL)和巨噬细胞集落刺激因子(M-CSF)联合应用诱导体外骨髓细胞培养系统中,转化生长因子(TGF-β)对破骨细胞分化的影响。方法:选用小鼠M-CSF依赖性非附着性骨髓细胞,在含有25 ng/m l sM-CSF和30 ng/m l sRANKL的α-MEM培养液中进行培养,比较加入和不加入1 ng/m lTGF-β1培养4、9 d后,所形成的抗酒石酸酸性磷酸酶染色(TRAP)阳性多核细胞的数目和骨吸收面积。结果:小鼠骨髓细胞在RANKL和M-CSF共同作用下可形成TRAP阳性多核巨细胞,并具有骨吸收功能。加入TGF-β后,破骨样细胞的数目及骨吸收面积显著增加。结论:TGF-β对RANKL和M-CSF诱导的破骨细胞的分化及其功能的促进作用,可能为炎症状态下发生过度骨吸收的机制之一。
OBJECTIVE: To investigate the effect of transforming growth factor-beta (TGF-β) on the osteoclastogenesis in osteoblasts induced by the combined use of RANKL and M-CSF. The impact of differentiation. Methods: Mouse M-CSF-dependent non-myeloid bone marrow cells were selected and cultured in α-MEM containing 25 ng / ml sM-CSF and 30 ng / ml sRANKL. Compared with and without addition of 1 ng / m The number of tartrate-resistant acid phosphatase-stained (TRAP) -positive multinucleated cells and bone resorption area formed after 4 and 9 days of culture of TGF-β1. Results: Murine bone marrow cells could form TRAP positive multinucleated giant cells under the combined action of RANKL and M-CSF, and had bone resorption function. After adding TGF-β, the number of osteoclast-like cells and bone resorption area increased significantly. Conclusion: The effect of TGF-β on the differentiation and function of osteoclasts induced by RANKL and M-CSF may be one of the mechanisms of excessive bone resorption in inflammatory state.