Comparison of Three Different Techniques of Human Sperm DNA Isolation for Methylation Assay

• 作 者: Hong-fang YUAN Martin KUETE Li
• 机 构: CenterofReproductiveMedicine,FamilyPlanningWomenandChildrenServiceCenterofZengDuDistrict
• 出 处: 华中科技大学学报：医学英德文版
• 发表日期: 2015年6期
• 关键词: DNA甲基化 提取技术 人精子 检测 脱氧核糖核酸 异硫氰酸胍法 凝胶电泳分析 遗传物质 human sperm DNA isolation modifie
• 基金项目: This project was supported by the National Natural Science Foundation of China （No. 81370755）.

Human sperm DNA is an important genetic and epigenetic material, whose chromatin structure differs from that of somatic cells. As such, conventional methods for DNA extraction of somatic cells may not be suitable for obtaining sperm DNA. In this study, we