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目的：对23例原发性肝细胞肝癌组织中p53基因的5～8四个外显子可能发生的点突变进行检测分析。方法：通过聚合酶链反应／单链构象多态性分析技术。结果：13例（56.50％）癌变组织中检测到点突变的发生，第7及5外显子的突变率分别高达47.83％、43.47％，11例癌变组织中同时出现两个外显子以上的多位点突变。结论：提示p53基困的突变在原发性肝细胞肝癌中可能是细胞内一系列基因调变至癌过程中的一个关键。同时，p53基因第5外显子可能存在突变热点。
Objective: To analyze the possible point mutations in 5-8 exons of p53 gene in 23 cases of hepatocellular carcinoma. Methods: Polymerase chain reaction/single strand conformation polymorphism analysis techniques. RESULTS: Point mutations were detected in 13 cases (56.50%) of cancerous tissues. The mutation rates of exons 7 and 5 were as high as 47.83% and 43.47%, respectively. Two exons were present in 11 cases of cancerous tissues. Multiple site mutations. Conclusions: The mutation that indicates that p53 is trapped in the primary hepatocellular carcinoma may be a key factor in the intracellular regulation of a series of genes to cancer. At the same time, there may be mutation hot spots in exon 5 of p53 gene.