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本研究以红掌‘Alabama’幼苗为材料,克隆了2个b ZIP转录因子基因Aab ZIP1和Aab ZIP2。Aab ZIP1片段长度462 bp,Aab ZIP2片段长度为369 bp,两基因核苷酸序列的相似度为46.97%,推定的氨基酸序列相似性为31.17%。Blastx发现两个基因均具有亮氨酸拉链的保守结构域,具备b ZIP转录因子家族的基本特征。RT-PCR分析检测表明,低温处理后,Aab ZIP1基因为组成型表达,不响应低温胁迫;Aab ZIP2基因表达受低温诱导,其表达强度随着低温处理时间的延长而增强,Aab ZIP2可能在红掌的低温胁迫响应过程中起重要作用。
In this study, two b ZIP transcription factor genes, Aab ZIP1 and Aab ZIP2, were cloned from the seedlings of ’Anthurium’ Alabama ’. The Aab ZIP1 fragment was 462 bp in length and the Aab ZIP2 fragment was 369 bp in length. The similarity of the two nucleotide sequences was 46.97%, and the putative amino acid sequence similarity was 31.17%. Blastx found that both genes have a conserved leucine zipper domain with the basic features of the b ZIP transcription factor family. RT-PCR analysis showed that Aab ZIP1 gene was constitutively expressed and did not respond to low temperature stress after low temperature treatment. The expression of Aab ZIP2 gene was induced by hypothermia, and the expression intensity of Aab ZIP2 increased with the prolongation of low temperature treatment. The Aab ZIP2 gene may be red Palm cold stress plays an important role in the response process.