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采用基因芯片技术和GO Term分析研究了黑豆萌芽中抗氧化活性相关基因的表达,并通过RT-PCR进行验证。结果表明:对比0.5和5.0 cm黑豆萌芽的差异表达基因,在芽长0.5 cm的萌芽中发现了10个与抗氧化活性相关的基因显著高表达,他们属于GO:0006979(response to oxidative stress,氧化压力响应):BE823689.1_567,GMFL01-02-F14-R_381,GMFL01-03-G22-R_364,GMFL01-14-M12-R_553,GMFL01-51-M23-R_265,AW757007.1_297,AW761420.1_260,BI788389.1_501,BQ273202.1_332,GMFL01-10-I14-F_701,这些基因主要控制合成热休克蛋白和抗氧化酶类,与细胞内的氧化代谢平衡密切相关;RT-PCR结果与基因芯片试验结果一致,证明了基因芯片结果的准确性和可重复性。
The expression of antioxidant-related genes in black bean sprouts was studied by gene chip technology and GO Term analysis and verified by RT-PCR. The results showed that compared with 0.5 and 5.0 cm black bean sprouting differentially expressed genes, ten genes related to antioxidant activity were found to be highly expressed in buds 0.5 cm in length. They belong to GO: 0006979 (response to oxidative stress, Pressure response): BE823689.1_567, GMFL01-02-F14-R_381, GMFL01-03-G22-R_364, GMFL01-14-M12-R_553, GMFL01-51-M23-R_265, AW757007.1_297, AW761420.1_260, BI788389. 1_501, BQ273202.1_332 and GMFL01-10-I14-F_701. These genes mainly control the synthesis of heat shock proteins and antioxidant enzymes, and are closely related to the balance of intracellular oxidative metabolism. The results of RT-PCR are in good agreement with those of gene chip test Gene chip results accuracy and repeatability.